Spatial Transcriptomics Services
for Every Discovery
Signios Bio offers Visium CytAssist and Xenium in Situ services as a 10x Genomics-certified provider. Generate high-resolution spatial gene expression data from FFPE or fresh-frozen tissue to map gene expression patterns across your tissue samples.
10x Genomics Spatial Services
We offer multiple options for your spatial projects with 10x Genomics Visium and Xenium. Review the two services below with more details.
10x Genomics-certified provider
| Visium CytAssist Services | Xenium Services | |||
|---|---|---|---|---|
| Slide Options | HD 3’ Slide | HD WT | Small Panel | Prime 5K |
| Coverage | Whole transcriptome – 3’ based (Detects >18,000 genes with enhanced UMI capture and deep sequencing) | Whole transcriptome – Probe-based (Detects >18,000 genes with enhanced UMI capture and deep sequencing) | Sensitive Targeting of 50-480 genes with custom gene add-ons | Sensitive Targeting of 5,000 genes with custom gene add-ons |
| Customized Genes | No, but kit is whole transcriptome | Design custom spike-in probes for detecting alternative genes, viral, or foreign transcripts | Customizable add-on up to 480 genes | Customizable add-on up to 100 genes |
| Protein Staining | IF staining services are available | IF staining services are available | Add-on panel of 27 proteins | Coming Soon |
| Sample Compatibility | Fresh-frozen OCT | FFPE, fresh-frozen, fixed-frozen | Fresh-frozen, FFPE (imaging optimized) | Fresh-frozen, FFPE (imaging optimized) |
| Species | Species agnostic | Human and Mouse | Human | Human |
| Resolution | Near single-cell with segmentation | Near single-cell with segmentation | Single-cell (direct assignment) | Single-cell (direct assignment) |
| Workflow | Sequencing + visualization tools | Sequencing + visualization tools | In situ imaging + segmentation | In situ imaging + segmentation |
| Best Use Case | Near single-cell mapping using poly A-based capture. Great for discovering novel genes of interest | Near single-cell mapping using Probe-based chemistry. Customize spike in probes to look at viruses or additional genes of interest | Targeted spatial transcriptomics with custom or pre-designed gene panels for focused hypothesis-driven research | High-plex imaging-based spatial transcriptomics for comprehensive cell typing and spatial gene expression analysis |
Whether your research calls for high-plex targeted analysis or unbiased transcriptome-wide profiling, our team will help determine the right platform or combination for your project.
Need a detailed technical comparison of these platforms?
Read our latest blog post: 10x Visium HD vs. Xenium Prime: Advanced Deep Dive
Sample Preparation & Tissue Processing Services
Our team supports all sample preparation and pre-treatment steps to optimize slide placement, ensure data quality or layering for protein analysis.
Tissue Sectioning
Ship Fresh frozen, OCT, or FFPE blocks for processing
H&E Staining
Pre and post-H&E staining services available
TMA Construction
Enable multiplexing of tissues. Up to 8 samples per Xenium slide or as many cores within the capture region of Visium.
Pre-QC
All samples undergo RNA quality assessment (DV200 ≥ 30%) before proceeding to ensure optimal results.
IF Staining
Prestaining of Visium slide to layer imaging with spatial data for deeper analysis
Pre-Placed Visium or Xenium Slide
Our team can accept preplaced slides for downstream processing.
Spatial Transcriptomics Workflows
Visium Whole-Transcriptome Spatial Gene Expression With CytAssist placement
Visium spatial captures gene expression across entire tissue sections at the whole-transcriptome scale. CytAssist automates probe transfer and library preparation to achieve consistent capture performance and robust gene detection across FFPE, fresh-frozen, and fixed-frozen tissue.
- Unbiased, whole-transcriptome discovery for novel biology exploration
- Novel isoform detection and comprehensive gene-regulatory mapping
- Broad species applicability, including non-model organisms
- Large-cohort analytical pipelines and retrospective studies
- Detection of novel transcripts using custom probes
- Archival sample flexibility with FFPE tissue sections
Xenium In Situ – Targeted Spatial Transcriptomics at Subcellular Resolution
The 10x Genomics Xenium platform delivers high-plex, imaging-based spatial transcriptomics at subcellular resolution with nanometer precision. Using padlock probe chemistry and rolling circle amplification, Xenium maps hundreds to thousands of RNA targets in situ within individual cells—without tissue dissociation or sequencing. Signios Bio provides full Xenium spatial transcriptomics services, including panel selection consultation, sample processing, instrument runs, and data analysis.
- Reliable low-abundance transcript detection across a range of expression levels
- Direct cell assignment without computational segmentation artifacts
- Xenium Explorer visualization for immediate, on-instrument data exploration
- Multiplexed protein and RNA co-detection on the same tissue section
- Custom and pre-designed gene panels for hypothesis-driven research
- Subcellular resolution for precise localization of transcripts within cells
Bioinformatics & Data Analysis
Bioinformatics & Analysis — Space Ranger alignment, gene expression calling, and initial clustering for Visium; Xenium Explorer visualization and downstream spatial analysis for Xenium; advanced filtering, cell typing, and interactome analysis available for both platforms.
Data Visualization & Delivery — Explore results via our MAnGO interactive portal; receive publication-ready spatial feature plots, heatmaps, and cluster maps
Interested in Spatial services? Contact our scientific team to discuss the best option for your research goals and the available sample material.
Applications Across Disciplines
Spatial transcriptomics offers versatile insights across a range of research areas by combining high-resolution gene expression profiling with preserved tissue architecture:
Disease Area
Application
Disease Area
Oncology
Application
Uncover tumor heterogeneity, localize immune cell infiltration zones, and identify stromal interactions to guide the development of targeted therapies
Disease Area
Neuroscience
Application
Map transcriptional programs within precise brain subregions to study neural circuit function and neurological disease mechanisms
Disease Area
Immunology
Application
Profile spatial cytokine and chemokine landscapes in lymphoid or inflamed tissues to characterize immune microenvironment dynamics
Disease Area
Developmental Biology
Application
Visualize gene expression patterns during embryogenesis and organogenesis to gain insights into tissue patterning
Disease Area
Regenerative Medicine & Tissue Engineering
Application
Characterize native tissue architecture to inform scaffold design and cell-based therapies
Disease Area
Inflammatory Disease
Application
Differentiate active versus quiescent zones in fibrotic or inflammatory lesions to pinpoint pathogenic niches
This broad applicability makes spatial transcriptomic analysis a powerful tool for academic researchers and biotech teams seeking to link molecular insights with histological context.
Resources & Insights
White Paper
Maximize Discoveries with Spatial Transcriptomics
A concise overview of spatial transcriptomics and its key applications in developmental biology, neuroscience, cancer research, and regenerative medicine.
Case Study & On-Demand Webinar
Lung Cancer Research with Spatial Transcriptomics
A detailed case study with Dr. Mukesh Nyati of the University of Michigan, plus an in-depth, on-demand webinar examining spatial transcriptomic analysis of lung tumors.
Webinar
Spatial Transcriptomics in Traumatic Brain Injury
Explore altered gene expression in a TBI murine model with Professor Michelle H. Theus of Virginia Tech, Dr. Caroline De Jager, and Marco Corbo, including sample-prep tips, data-integration approaches, and key biological insights.
Why Researchers Choose Signios Bio
Signios Bio stands out as a trusted spatial transcriptomics service provider and 10x Genomics Certified Service Provider (with direct access to 10x Genomics technical support) for both Visium and Xenium, offering:
End-to-end support
From initial consultation through data interpretation, so you never worry about handoffs or delays
Rapid turnaround
Complete spatial transcriptomics projects in 3–4 weeks, or opt for data-only analysis in as little as 1-2 weeks
Secure, fast data pipeline
US-based lab, encrypted file transfer, and fully analyzed data to meet tight timelines
Flexible processing
With block cutting and H&E services, we support any sample material through spatial workflows and simplify logistics with all services in one location
Expert bioinformatics analysis
Integrating spatial transcriptomics with scRNA-seq or immune profiling to deliver tailored, publication-ready insights
Interactive visualization portal
MAnGO for exploring spatial feature plots, heatmaps, and cluster maps, plus publication-quality figures included in every report
Ready for Signios to Help with Your Next Project?
Discuss your spatial transcriptomics needs with our expert team and receive a personalized quote.