US Based · Immune Profiling Services
Map the Immune Repertoire with Precision
Profile the immune repertoire with Signios Bio’s TCR and BCR sequencing services. Industry-leading single-cell and bulk capture chemistries from 10x Genomics™, Takara™, and Cellecta™ for high-resolution clonotype discovery and immune repertoire profiling in 2-4 weeks.
Immune Profiling Services
Choose Your Repertoire Sequencing Strategy
Choose between single-cell resolution or bulk repertoire analysis options to study clonotype changes across all sample types.
Full-Length TCR & BCR Services
Unbiased profiling using Takara™ SMARTer 5' RACE to capture full-length TCR and BCR sequences. Optimized for high-quality RNA, PBMCs, whole blood, and fresh-frozen tissue.
CDR3 TCR & BCR Services
High-throughput clonotype analysis using Cellecta™ DriverMap™. Comprehensive 11-chain TCR and BCR captures to evaluate the entire repertoire.
FFPE TCR & BCR Services
TCR and BCR repertoire analysis from archived or degraded specimens using specialized Cellecta™ chemistries. Compatible with RNA (DV200 > 20%) and DNA inputs.
Single-Cell VDJ Service
Analyze gene expression along side expressed TCR and BCR chains, uncover chain pairs, lineage tracing and phenotype tracking using 10x Genomics™ single cell 5' plus VDJ chemistry.
The Signios Advantage
Superior Workflow Performance & Technical Excellence
From sample extraction through final bioinformatic analysis, our optimized protocols maximize clonotype capture, chain-pairing accuracy, and sequencing depth across every immune profiling project.
End-to-End Service Support
Integrated workflows starting with sample preparation and QC, library preparation aligned to your study design and samples, sequencing, and comprehensive analysis.
Multi-Capture Library Preparation
Comprehensive options featuring Takara™ SMARTer 5′ RACE, Cellecta™ DriverMap™, and 10x Genomics™ Chromium 5′ v2 chemistries to meet your analysis goals.
Broad Species Captures
Validated primers for Human, Mouse, Rat, and Non-Human Primate (NHP) for comprehensive TCR (αβ and γδ) and BCR (heavy/light) chain characterization.
Unrivaled Input Sensitivity
Low input protocols supporting >100 sorted T cells, fine-needle aspirates (FNA), and highly degraded clinical FFPE tissue specimens (DV200> 20%).
Precise Clonal Tracking
Enables precise tracking of clonal expansion, persistence, and contraction across multi-timepoint clinical studies or treatment regimens.
US-Based Services & Security
All immune profiling services are performed in-house at our Foster City, CA facility ensures rapid turnarounds and direct chain-of-custody.
Advanced Bioinformatics
Seamless reporting for clonotype quantification, nucleotide and amino acid sequences, V(D)J allele segment usage aligned with publication-ready figures.
Expert Scientific Support
PhD-level scientific oversight for every project. From initial study design and chemistry selection to sample QC and post-sequencing data walkthroughs.
The Signios Partnership
Laboratory Workflow & Quality Assurance
A transparent, five-step workflow designed to preserve sample integrity and ensure high-confidence immune repertoire data generation.
Step 1
Our scientists provide dedicated guidance to align our workflows with your sample species, material quality, and research objectives.
Step 2
Register your samples via our streamlined customer portal. Schedule a local SF Bay Area courier pick-up or ship directly to our CA lab when ready.
Step 3
We extract RNA/DNA and provide formal Qubit or high-resolution TapeStation metrics for your review, ensuring complete transparency and customer sign-off before library construction.
Step 4
Libraries are molecularly indexed, QC'd, and pooled to ensure high-fidelity target read outputs from the advanced Illumina NovaSeq X Plus platform.
Step 5
Secure delivery of raw FASTQ files and comprehensive QC reports via cloud transfer. Turnkey bioinformatics options are available for annotated clonotype quantification and diversity tracking.
Our scientists provide dedicated guidance to align our workflows with your sample species, material quality, and research objectives.
Register your samples via our streamlined customer portal. Schedule a local SF Bay Area courier pick-up or ship directly to our CA lab when ready.
We extract RNA/DNA and provide formal Qubit or high-resolution TapeStation metrics for your review, ensuring complete transparency and customer sign-off before library construction.
Libraries are molecularly indexed, QC'd, and pooled to ensure high-fidelity target read outputs from the advanced Illumina NovaSeq X Plus platform.
Secure delivery of raw FASTQ files and comprehensive QC reports via cloud transfer. Turnkey bioinformatics options are available for annotated clonotype quantification and diversity tracking.
Beyond the FASTQ
Comprehensive Bioinformatics
Automated bioinformatics pipelines transition your project from raw sequencing data to biological insight. We tailor industry-standard workflows to your study, covering everything from bulk clonotype tracking to single-cell immunophenotyping and paired-chain TCR/BCR analysis.
Deliverables Include
- FASTQ files
- Data QC report
- Alignment files
- Clonotype frequency
- Nucleotide and amino acid sequences
- V & J gene usage
- Phylogenetic analysis
Top Clonal Proportion
Rare Clonal Proportion
Dendogram
Spectratype Plots
Shannon Diversity
Circos Plot
Applications of Immune Profiling Sequencing
Our high-level TCR-Seq and BCR-Seq services support a broad range of multi-disciplinary applications, including:
Biomarker & Target Discovery
Comparing immune repertoires between experimental cohorts to isolate signature disease-specific or therapeutic-responsive clones.
Immuno-Oncology & TIL Characterization
Evaluating tumor-infiltrating lymphocyte (TIL) expansion, tracking systemic clonal distribution, and assessing microenvironment engagement.
Vaccine Development & Antibody Discovery
Mapping structural somatic hypermutation patterns, class-switch recombination events, and specific isotype distribution profiles following antigen challenge.
Autoimmune & Infectious Disease
Monitoring abnormal clonal dominance patterns, tracking individual cell activation profiles, and measuring response pathways across disease states.
Cell Therapy Tracking (CAR-T / TCR-T)
Monitoring the engraftment, absolute survival persistence, and longitudinal systemic expansion dynamics of engineered cell therapies in clinical models.
Resources & Insights
SERVICE OVERVIEW | TECH SHEET
Bulk and Single-Cell TCR Services
Explore our high-throughput workflows. Discover how we map adaptive immune repertoires, pair alpha/beta chains, and track functional cellular phenotypes.
SERVICE OVERVIEW | TECH SHEET
Bulk and Single-Cell BCR Services
Explore our high-throughput workflows. Discover how we map adaptive immune repertoires, pair heavy/light chains, and track functional cellular phenotypes.
Technical Guide
TCR Seq QC Standards
Learn how our laboratory utilizes Qubit and TapeStation systems to evaluate your starting material (RIN and DV200 metrics) to guarantee optimal library prep before sequencing begins.
Frequently Asked Questions
Everything you need to plan your project
How do I choose the right service?
- Bulk (Takara/Cellecta): Best for cost-effective, deep tracking of clone frequencies and aggregate diversity.
- Single-Cell (10x Genomics): Best for linking exact paired chains (αβ or heavy-light) to individual cell phenotypes.
- Spatial: Best for mapping immune architecture directly within tissue sections.
What species are supported?
We specialize in Human, Mouse, Rat, and NHP.
- Takara: Supports Human, Mouse, Rat, and NHP.
- 10x Genomics: Supports Human, Mouse, and Rat.
- Cellecta: Primarily engineered for Human and Mouse.
Which bulk kit should I choose?
- Takara SMARTer: Best for full-length V(D)J sequencing of variable regions.
- Cellecta Full-Length: Complete V(D)J variable region sequencing.
- Cellecta CDR3: Best for absolute digital clonotype quantification and rare subset analysis.
What are the input requirements?
- Purified RNA: 20 ng – 1,000 ng total RNA (RIN >6 preferred). Working volume 15 µl
- Cell Suspensions (Bulk): 50,000 cells recommended; 100 cells minimum.
- Single-Cell: 1 – 1,000,000 cells (>85% viability).
- FFPE Tissues: 5–8 slides/core curls (10 µm thickness) with DV200 > 20%.
What is your turnaround time?
- Standard: 3–4 weeks from QC approval.
- Expedited (Bulk only): 10–12 business days.
Where is sequencing performed and on what platforms?
All work is done in-house at our Foster City, CA facility using Illumina NovaSeq X Plus and MiSeq i100 platforms.
What quality control (QC) do you provide?
We perform rigorous Qubit and TapeStation validation on all incoming samples and provide formal reports for your sign-off before library construction begins.
Explore Related Sequencing Solutions
High-resolution transcriptomics to resolve individual cellular heterogeneity.
High-sensitivity, scalable gene expression profiling and transcriptome mapping.
Resolving complex structural variations and full-length RNA isoforms.
High-plex protein profiling for functional multi-omic integration.
Start your immune profiling project
Tell us about your samples and research goals. A PhD scientist will recommend the ideal TCR or BCR sequencing workflow and provide a tailored quote within one business day.
- US-based, in-house laboratory in Foster City, CA
- Bulk & single-cell TCR/BCR repertoire sequencing
- End-to-end workflow with bioinformatics support
Request a quote