US Based · Single-Cell Sequencing Services
Multi-Platform Single-Cell Sequencing Services
End-to-end single-cell workflows utilizing 10x Genomics, BD Rhapsody, Takara, and PacBio chemistries. From our Foster City, SF Bay Area lab, we provide transcriptomic and multi-omic profiling across diverse sample types with fast turnaround.
Precision Single-Cell Services
Multi-Platform Workflows for High-Resolution Biology
10x Genomics Single Cell Services
High-throughput droplet-based profiling for samples with >100k starting cells screening for gene expression, Epi Multiome, FLEX, Immune profiling, and CITE-Seq chemistries.
BD Rhapsody™ Single-Cell Services
Microwell-based single-cell analysis with gentle, gravity-driven cell partitioning for high capture efficiency and low doublet rates. Ideal for fragile, large, or low-input samples.
Takara Plate-Based Single Cell Services
Ultra-sensitive, plate-based full-length gene expression or full-length TCR using SMART-Seq chemistries. Best for FACS-sorted low-abundance cell suspensions, laser capture microdissection (LCM), fine-needle aspirates (FNA), and rare cell populations.
PacBio Single-Cell Services
Long-read single-cell transcriptomics leveraging Kinnex workflows to sequence complete, full-length transcripts for novel isoform discovery, alternative splicing characterization, gene fusion detection, and structural variant mapping at a single-cell level.
Not sure which service fits your project? Our scientists provide complimentary technical consultations to help you select the optimal workflow based on your sample quality, storage conditions, and research objectives. Read more about each of our services by following the links above or shortcut to explore Technical Documents and FAQ sections below.
The Signios Advantage
Superior Workflow Performance & Technical Excellence
Our end-to-end workflows feature expert sample preparation utilizing automated tissue dissociation with Miltenyi gentleMACS™, FACS cell sorting, and dead cell removal to guarantee the highest data quality. From tissue-specific dissociation workflows through final custom analysis, our optimized services maximize cell capture, target recovery and sequencing coverage across every project.
End-to-End Solutions
Experience a seamless transition from automated cellular extraction and FACS cell sorting to multi-stage QC and interactive bioinformatics. Having a single trusted partner manage the entire process reduces logistics and technical data variability.
Multi-Platform Flexibility
Choose between droplet, microwell, multi-well plate, short-read, and long-read setups to fit your study goal. Our scientists guide you in choosing the exact capture chemistry required for your target cell counts and transcript coverage.
Automated Prep & Debris Removal
We utilize Miltenyi gentleMACS™ automated tissue dissociator and FACS cell sorting to safely remove dead cells, debris, and sample impurities. Custom upstream handling preserves delicate cell surfaces and avoids stress-induced transcriptional artifacts for pristine data.
Broad Sample Compatibility
Our validated certified services for single-cell and single-nuclei support hard-to-dissociate tissues and challenging samples. We tailor each workflow to ensure consistent results from complex, “difficult” samples like brain, liver, kidney, or solid tumors.
Local Expertise & Security
All single-cell services, fluidics, partition capture, and sequencing are performed entirely in-house at our Foster City, CA facility with fast turnaround times.
Expert PhD Support
Every single-cell project is overseen from study design through library preparation by PhD-level scientists. Upfront consultation and QCs ensure cell counts, viability thresholds, antibody labeling strategies, and pilot goals are successfully met.
The Signios Partnership
Laboratory Workflow & Quality Assurance
A transparent, five-step process designed to ensure sample integrity and data accuracy.
Step 1
We align cell inputs, capture technology, multiplexing strategies, and required read depths to match your biological goals.
Step 2
Register samples via our streamlined customer portal. Schedule local Bay Area pick-up or prioritize cold-chain shipping.
Step 3
We execute automated dissociation or single-nuclei extractions, delivering count and cell viability checkpoints prior to chip loading.
Step 4
Single cells are partitioned into droplets or microwells, barcoded, and sequenced on high-throughput platforms.
Step 5
Secure cloud delivery of FASTQ matrices alongside publication-grade cluster figures and custom multi-omic integrations.
We align cell inputs, capture technology, multiplexing strategies, and required read depths to match your biological goals.
Register samples via our streamlined customer portal. Schedule local Bay Area pick-up or prioritize cold-chain shipping.
We execute automated dissociation or single-nuclei extractions, delivering count and cell viability checkpoints prior to chip loading.
Single cells are partitioned into droplets or microwells, barcoded, and sequenced on high-throughput platforms.
Secure cloud delivery of FASTQ matrices alongside publication-grade cluster figures and custom multi-omic integrations.
Beyond the FASTQ
Advanced Single-Cell Bioinformatics
Our dedicated bioinformatics pipeline handles the unique computational demands of single-cell and spatial data. Utilizing best-in-class tools (including Cell Ranger, Seurat, and other tools), we transition complex count matrices into clear, interactive biological landscapes.
Deliverables Include
- FASTQ files
- Illumina Data QC report
- Alignment files (BAM)
- CellRanger outputs of sequencing QC metrics
- CellRanger outputs of gene expression and heatmap visualization
- CLOUPE file for use in LOUPE browser
- Advanced QC via Seurat
Scoring Heat Map
Delta Distribution
Clonotype Expansion
Cell Type Annotation
Dimensionality Reduction (UMAP / t-SNE) Cluster Plots
Cell-Type Annotation & Subpopulation Distribution
TCR/BCR Clonal Frequency Repertoire Maps
Cell-to-Cell Interaction
Pathway Analysis
V-J Circos Plot
Applications of Single-Cell Sequencing
Our single-cell sequencing services support a broad range of research applications, including:
Tumor Microenvironment (TME) Profiling
Uncover rare immune-evading cell populations and immune cell infiltration inside solid tumors.
Immune Repertoire Mapping
Trace exact BCR/TCR clonotypes and heavy-light chain pairings during disease states or therapeutic interventions.
Therapeutic Target Discovery
Identify distinct cellular subpopulations driving drug resistance or responding to targeted mechanisms of action.
Resources & Insights
Blog
Droplet, Microwell, or Plate? A Decision Tree Guide to Single-Cell Platforms
A comprehensive technical breakdown to help you select the ideal single-cell chemistry and sequencing depth based on your specific sample type and viability metrics.
SERVICE OVERVIEW | TECH SHEET
Single Cell Solutions at Signios Bio
Download our complete technical menu detailing precise cell viability thresholds, sample count matrices, and cold-chain shipping requirements for our SF Bay Area lab.
Blog
Single-Cell Sequencing: Applications, Methods, and Insights
Explore the core methodologies driving single-cell discovery and learn how to select the right application for your translational research.
Frequently Asked Questions
Everything you need to plan your project
How do I initiate a single-cell project?
Click our Request a Quote link or reach out through our portal. Our specialists will schedule an introductory technical call to detail sample logistics and provide your project quote.
What single cell services do you offer?
We offer comprehensive single-cell services leveraging the 10x Genomics Chromium platform, the BD Rhapsody™ Single-Cell Analysis System, 96-well plate-based Takara SMART-Seq (Plate-Seq), and PacBio Kinnex library preps with sequencing. There are multiple library prep options for each service to analyze gene expression, ATAC, immune profiling, and antibody surface markers.
What bioinformatics options are provided?
Our single-cell analysis includes standard alignment and quality evaluation (Cell Ranger), advanced cluster mapping and cell-type identity annotation (Seurat/Scanpy), or custom multi-omic integration pipelines.
What protocols are supported for each cell state?
We support custom workflows optimized for fresh/FACS cells, cryopreserved cells, fresh tissue nuclei prep, frozen tissue nuclei input, and fixed tissue profiles (Chromium Flex).
What are the starting cell requirements?
Our portfolio of single-cell services covers 1 to 60,000 cell captures per sample depending on the platform selected.
Can you handle fixed cells or tissue slides?
Yes, you can send flash-frozen tissues or cryopreserved cells for our team to complete the fixing steps, or you can send fixed cells/nuclei/tissues directly. Fixed cells and tissues are compatible with the probe-based 10x Genomics Chromium Flex protocol.
What types of tissue and sample formats does your laboratory specialize in?
Our laboratory is optimized to handle a vast array of complex sample formats to ensure high-quality cell suspensions, specializing in:
- Primary Human & Animal Tissues: Comprehensive experience with brain (neuroscience), tumor biopsies (oncology), lung, liver, kidney, and heart matrices.
- Liquid Biopsies & Suspension Cells: Expert processing of PBMCs, B-cells, T-cells, and bone marrow aspirates for immunology and vaccine research.
- Challenging & Clinical Samples: Specialized protocols for flash-frozen tissues (Single-Nuclei Sequencing), low-viability archival samples, and fragile primary cultured cells.
Explore Related Sequencing Solutions
Start your single-cell sequencing project
Tell us about your samples and research goals. A PhD scientist will recommend the right single-cell workflow and provide a customized quote within one business day.
- US-based, in-house laboratory at Foster City, CA
- 10x Genomics Chromium, BD Rhapsody & Takara workflows
- scRNA-Seq, Immune Profiling, CITE-Seq, ATAC & Multiomics
- Expert sample prep, bioinformatics & publication-ready results
Request a quote