Long-Read Sequencing for Genome Assembly and Transcriptome Analysis
Seamless long-read sequencing support—from assembly to RNA insights, powered by expert bioinformatics.
Long-Read Sequencing Solutions for Complete Genomic Research
Signios Bio offers advanced long-read sequencing services to academic researchers and biotechnology companies seeking accurate, publication-ready genomic data. Using PacBio HiFi technology, we deliver precise, highly reads that resolve complex genomic regions, characterize structural variants, and capture full-length transcripts — supporting projects in human, plant, microbial, and animal genomics.
Why Long-Read Sequencing Matters
Long-read sequencing reveals insights that short-read methods often miss. Our PacBio HiFi sequencing produces read lengths ranging from 10–25 kb with ~99.9% Q30 accuracy, enabling you to:
- Assemble chromosome-scale genomes for human, plant, and animal research.
- Detect all variant types, including SNVs, Indels, and large structural variants.
- Resolve repetitive, polyploid, and GC-rich regions.
- Fully characterize isoform diversity and discover novel transcripts.
- Profile methylation and epigenetic patterns without additional chemical treatment.
Our PacBio HiFi Long-Read Sequencing Workflows
De Novo Genome Assembly and Annotation
Short reads limit assembly contiguity and gene annotation accuracy. PacBio HiFi sequencing enables the assembly of contiguous, accurate genomes, resulting in complete genome builds with comprehensive gene and isoform annotations — ideal for species with complex or polyploid genomes.
Comprehensive Human Variant Detection
Short-read platforms often miss large SVs or complex variants. HiFi sequencing captures all variant types, even in difficult regions, producing high-confidence SNV, Indel, and SV calls with haplotype phasing.
Full-Length RNA Sequencing
Isoform diversity and fusion transcripts are difficult to resolve with fragmented reads. PacBio RNA sequencing offers comprehensive transcript coverage in both bulk and single-cell samples, enabling isoform-level resolution, the detection of fusion genes, and the characterization of non-coding RNAs across human, plant, and animal samples.
Integrated Bioinformatics for Long-Read Data
Our bioinformatics team provides end-to-end support, ensuring your project moves seamlessly from raw data to actionable insights:
Why Partner with Signios Bio
- Scientist-to-scientist consultation for optimized project design and sample selection.
- Expert sample processing for maximum data yield and quality.
- Extensive capabilities for standard, advanced, and custom analysis.
- Stringent quality controls at every step of sequencing and analysis.
Get Started
Whether your project involves human, plant, or animal genomes, our long-read sequencing services provide the accuracy and depth your research demands.
Frequently Asked Questions
1. What is PacBio HiFi long-read sequencing?
A next-generation sequencing technology producing long, highly accurate reads using circular consensus sequencing (CCS), enabling detailed genome and transcriptome analysis.
2. What applications benefit from long-read sequencing?
Genome assembly, structural variant detection, isoform discovery, methylation profiling, and targeted sequencing in human, plant, and animal research.
3. How much input DNA is required?
Typically, 500ng–10 µg of high-molecular-weight DNA, though requirements vary by application.
4. Do you provide bioinformatics support?
Yes — all projects include consultation and optional complete bioinformatics analysis, from raw data to publication-ready results.
Start your Genomic Discovery
Accurate long-read sequencing for human, plant, and animal genomes