The Signios blog
Insights from the lab
Protocols, application notes, and perspectives on NGS and multi-omics from our team of PhD-level scientists.
Navigating the spatial biology landscape can feel like standing in front of a massive soundboard, trying to balance dozens of sliders at once.
Scientific Affairs Team,
Signios
Choosing the Right library prep for Low-Input RNA-Seq
In transcriptomics, sample quality often dictates experimental success. When working with ultra-low input samples, you rarely have the luxury of a “perfect” starting material....
Beyond the RIN Score: Why DV200 is the Only Metric That Matters for FFPE RNA Sequencing
If you work with clinical biobanks or archived human tissues, you know that Formalin-Fixed, Paraffin-Embedded (FFPE) tissue blocks are a scientific goldmine. They hold decades...
Don’t Waste Your Rare Cells: A Scientist’s Guide to Successful Sample Preparation for Ultra-Low Input RNA-Seq
When your entire experiment relies on a few hundred laser-captured cells, a rare circulating tumor cell population, or a handful of precious fluorescence-activated cell sorting...
Poly(A) Selection vs. Ribosomal Depletion: Which RNA-Seq Approach is Right for Your Project?
When designing a transcriptomics experiment, researchers focus deeply on biological variables: choosing the right time points, establishing robust controls, and ensuring sufficient biological replicates....
Why RIN Matters: How to Optimize Your RNA Quality for Successful mRNA-Seq
Every molecular biologist knows the unique anxiety of submitting samples for next-generation sequencing. You have spent weeks, perhaps months, culturing cells, dosing animal models, or...
The Spatial Biology Decision Matrix: Visium HD vs. Xenium Prime vs. Illumina StrataMap
Navigating the spatial biology landscape can feel like standing in front of a massive soundboard, trying to balance dozens of sliders at once. Every platform...