Transcriptomics Sequencing Services
Delivering high-quality expression data across bulk, single-cell, spatial, and long-read resolutions. Cost-effective, end-to-end transcriptomics from our SF Bay Area lab.
Transcriptomics Services
Bulk RNA, single-cell, and spatial insights for diverse research needs
Bulk RNA Sequencing
Services
High-quality, cost-effective gene expression profiling using Illumina and Takara workflows. Highly optimized for standard inputs, low-input samples, and clinical FFPE tissues.
Single-Cell RNA-Seq
Services
Uncover cellular heterogeneity and identify rare cell populations with high-throughput single-cell
transcriptomics.
Spatial Transcriptomics Services
Map gene expression architecture directly within tissue sections, preserving critical spatial context and morphological data.
PacBio Long-Read Transcriptomics
Accurately characterize complex isoforms, alternative splicing events, and gene fusions using full-length cDNA sequencing (Iso-Seq).
Not sure which service fits your project? Our scientists provide complimentary technical consultations to help you select the optimal workflow based on your sample quality and research objectives. Read more about our services below or use the shortcut to explore the Technical Documents and FAQ sections.
The Signios Advantage: Superior Workflow Performance & Technical Excellence
From sample extraction through final multi-omic analysis, our optimized protocols maximize transcript capture, sequencing depth, and coverage consistency across every project.
End-to-end Solutions
Integrated workflows spanning sample extraction, quality control, library preparation, sequencing, bioinformatics analysis, and reporting—all managed within a single laboratory.
Multi-Platform Profiling
Access a comprehensive toolkit featuring Illumina short-read, PacBio long-read, single-cell, and spatial profiling methods tailored to your study goals.
Unrivaled Sensitivity
Validated single-cell and low-input protocols support ultra-low-input RNA samples down to single-cell levels, as well as degraded FFPE specimens.
High Strand Specificity & Accuracy
Validated stranded RNA-Seq workflows enable accurate detection of antisense transcription, overlapping genes, splice variants, and fusion transcripts.
US-Based Services
All transcriptomics services are performed in-house at our Foster City, CA facility. No outsourcing or international shipping.
Expert Support
Every project is overseen by PhD-level scientists providing guidance on study design, platform selection, QC consultation, and data interpretation.
Scalable Sequencing Infrastructure
Powered by the latest Illumina NovaSeq X Plus and PacBio Sequel platforms for ultra-deep transcriptomic coverage and high throughput.
Fast Turnaround Times
Optimized protocols utilizing state-of-the-art liquid handlers for efficient sample processing and rapid data delivery.
The Signios Partnership: Laboratory Workflow & Quality Assurance
A transparent, five-step workflow designed to preserve sample integrity and ensure high-confidence transcriptomic data generation.
Step 1
Consultation & Study Design
Step 2
Order & Sample Submission
Step 3
Sample QC & Evaluation
Step 4
Library QC & Sequencing
Step 5
Data Delivery & Bioinformatics
Our scientists provide dedicated guidance to align our technical workflows (bulk, single-cell, spatial, or long-read) with your research objectives.
Register samples via our streamlined customer portal. Schedule a local Bay Area pick-up or ship directly when ready.
We provide formal Qubit, TapeStation, or Agilent Bioanalyzer reports for your review, ensuring full transparency and customer sign-off before library construction.
Libraries are QC’d and pooled to ensure high-fidelity data outputs from our advanced Illumina and PacBio sequencing platforms.
Secure delivery of FASTQ/BAM files and QC reports via SFTP or direct cloud transfer. Bioinformatics services are available for publication-ready transcriptomic interpretation.
Resources & Insights
SERVICE OVERVIEW TECH SHEET
Bulk RNA Sequencing Services
Learn about our end-to-end bulk RNA-Seq workflows, optimized for standard inputs, ultra-low-input samples, and challenging clinical FFPE tissues to maximize gene expression profiling.
SERVICE OVERVIEW TECH SHEET
Single-Cell RNA-Seq Services
Explore our high-throughput single-cell workflows. Discover how we map cellular heterogeneity, profile immune repertoires, and dissect complex microenvironments.
SERVICE OVERVIEW TECH SHEET
Spatial Transcriptomics Services
Download the technical overview of our spatial workflows to see how we map high-resolution gene expression directly within tissue sections while preserving structural morphology.
Beyond the FASTQ: Comprehensive Bioinformatics for Our Transcriptomics Services
Our automated bioinformatics pipelines transition your project from raw sequencing data to profound biological insight. We employ industry-standard workflows tailored to the resolution of your project—ranging from bulk differential expression to single-cell clustering and spatial alignment.
(A) Alignment Stats / Mapped Read Distribution
PCA & Correlation Plots
Correlation Plot
Heatmap
Expression Heatmaps & Volcano Plots
Tumor Microenvironment Deconvolution
Gene Networks
Dot Plot
Applications of Transcriptomics Sequencing
Our high-level transcriptomics services support a broad range of multi-disciplinary applications, including:
Differential Gene Expression
Comparing transcriptional profiles between experimental groups to isolate biomarkers.
Isoform & Splicing Analysis
Discovering novel transcripts, alternative splicing events, and gene fusions using long reads.
Oncology & Tumor Microenvironments
Uncovering the spatial distribution and cellular interactions of immune and cancer cells.
Immune Profiling
Understanding single-cell immune cell activation, receptor repertoires, and response pathways.
Drug Mechanism of Action
Evaluating how novel therapeutic compounds affect cellular pathways across various tissues.
Get Started with Your Project
Ready to accelerate your transcriptomic research? Contact our team today for a technical consultation. We will guide you through sample requirements, technology options, and library prep architectures aligned to your specific analysis goals.
Frequently Asked Questions
How do I choose between Bulk, Single-Cell, and Spatial Transcriptomics?
Bulk RNA-Seq provides a deep, cost-effective overview of average expression across tissues. Single-Cell mapping resolves cellular heterogeneity, and Spatial Transcriptomics retains exact morphological context. Our scientists can help guide your selection.
What is the typical turnaround time (TAT)?
Our standard turnaround time is 3–4 weeks from sample QC approval. We offer expedited services for select bulk RNA workflows down to 10–12 business days.
What sequencing platforms are utilized in-house?
We utilize the Illumina NovaSeq X Plus platform for high-throughput short-read processing (PE150), alongside advanced PacBio instruments for high-fidelity long-read (Iso-Seq) sequencing.
What sample types are compatible across your platforms?
Our services are validated for an extensive range of inputs: whole blood, fresh-frozen tissue, cultured cells, single-cell suspensions, and clinical FFPE blocks.
What are the sample requirements?
Requirements vary significantly by platform:
- Bulk RNA: Prefer >10–50ng purified RNA (RIN >5), or down to 10pg for ultra-low input.
- Single-Cell: High-viability (>80%) single-cell suspensions.
- Spatial: Fresh-frozen tissue or qualified FFPE blocks.
- PacBio Long-Read: High-integrity total RNA
(preferred RIN >7).
What type of quality control (QC) is provided?
All input samples undergo rigorous validation using Qubit and TapeStation architectures. Full transparency is provided via formal reporting before any platform run.
Explore Related Sequencing Solutions
High-resolution transcriptomics to resolve individual cellular heterogeneity.
High-sensitivity, scalable gene expression profiling and transcriptome mapping.
Resolving complex structural variations and full-length RNA isoforms.
Let’s discuss your transcriptomics project
Turn RNA into meaningful biological insights with scientist-led sequencing and bioinformatics