US Based – 10x Genomics Single Cell Services
Certified 10x Genomics Chromium single-cell workflows
A certified 10x Genomics Chromium service provider for 3’ Gene Expression, Epi Multiome, ATAC, CITE-Seq, OCM and Flex. Tissue dissociation, FACS sorting, comprehensive QCs, and data analysis all in one SF Bay Area lab.
Certified Workflows
Our 10x Genomics Chromium services
A certified suite of 10x Genomics workflows tailored to map complete cellular atlases, profile immune repertoires, and unlock epigenetic landscapes utilizing Chromium Next GEM technology.
Gene Expression
10x 3′ and 5′ GEX
High-throughput transcriptomic profiling to classify cell populations, find rare phenotypes, and build cellular atlases.
Fixed RNA Profiling
10x Flex
Probe-based chemistry that preserves fragile transcripts - ideal for FFPE blocks and clinical samples.
Epi Multiome
10x ATAC + GEX
Joint chromatin accessibility and gene expression from the same cell to map gene regulation and lineage trajectories.
Immune Repertoire
10x 5’ + VDJ
Paired full-length V(D)J TCR/BCR clonotypes with gene expression for cell-state & repertoire analysis.
CITE-Seq
10x GEX + Feature Barcoding
Transcriptomics, immune profiling & surface-protein immunophenotyping using BioLegend antibodies.
Multiplexing
OCM or Hashing
Sample hashing with Biolegend or On-chip multiplexing to pool samples into a single library prep reducing project costs.
Unsure which 10x Genomics workflow aligns with your sample type? Our PhD-level applications team provides complimentary study-design consultations to guide you through cell vs. nuclei inputs, multiplexing limits, and sequencing depth parameters. Read more about each of our services by following the links above or shortcut to explore Technical Documents and FAQ sections below.
The Signios Advantage
10x Genomics Technical Excellence
As a 10x Genomics Certified Service Provider, Signios Bio delivers tissue dissociation, library construction, and sequencing from our SF Bay Area facility. We optimize cell suspension quality, counting, and viability thresholds for single-cell data.
End-to-End Solutions
Tissue dissociation, Fixing, Nuclei isolation and/or FACS sorting services are available prior to multi-stage QC, library preparation, sequencing and bioinformatics. Processing in one lab to minimize technical variability.
Certified Chromium Workflows
All library prep options are available to run with 10x Genomics Chromium X/iX systems. Services are performed by certified operators following manufacturer specifications to ensure droplet stability and technical reproducibility.
Advanced Tissue Dissociation
Automated tissue dissociators with temperature-controlled handling yield single-cell suspensions while preserving cell surface markers and transcript profiles.
Optimized Nuclei Isolation
Mechanical and detergent-based isolation protocols extract nuclei from complex, fibrous, or flash-frozen clinical specimens, including archival tissue blocks and frozen tumors.
Sample Hashing
Multiplexing protocols use cell-surface oligos or sample barcodes to pool cohorts into a single sequencing lane, reducing library preparation costs and batch variations.
Deep Sequencing
Flexible sequencing coverage to reach cell depth and coverage based on analysis goal. Illumina NovaSeq X+ sequencing starting at 20k reads/cell.
The Signios Single-Cell Partnership
10x Laboratory Quality Control
A highly structured, five-step processing architecture engineered to protect your samples and maximize valid cell data capture.
Step 1
We align your specific biological goals with the proper 10x chemistry, target cell outputs, hashing strategies, and exact read depths.
Step 2
Coordinate same-day or overnight sample delivery to our Foster City facility. Local SF Bay Area couriers can pick up samples for rapid transport.
Step 3
Tissue dissociation, cell viability checks, FACS sorting, and single-nuclei isolation services are provided upstream of library prep. Every sample goes through comprehensive QC before loading.
Step 4
Cells are combined with barcoded Gel Beads to generate single-cell GEMs. Following library construction, samples are run on our NovaSeq X Plus system to hit targeted read depths.
Step 5
Receive raw, fully demultiplexed FASTQ files. Add-on options for Cell Ranger reporting and advanced analysis packages with publication-ready reports.
We align your specific biological goals with the proper 10x chemistry, target cell outputs, hashing strategies, and exact read depths.
Coordinate same-day or overnight sample delivery to our Foster City facility. Local SF Bay Area couriers can pick up samples for rapid transport.
Tissue dissociation, cell viability checks, FACS sorting, and single-nuclei isolation services are provided upstream of library prep. Every sample goes through comprehensive QC before loading.
Cells are combined with barcoded Gel Beads to generate single-cell GEMs. Following library construction, samples are run on our NovaSeq X Plus system to hit targeted read depths.
Receive raw, fully demultiplexed FASTQ files. Add-on options for Cell Ranger reporting and advanced analysis packages with publication-ready reports.
Beyond the FASTQ
Specialized 10x Genomics Bioinformatics
Our dedicated computational pipeline translates raw base calls into structured, interactive single-cell biology. Utilizing official 10x Genomics Cell Ranger tools seamlessly paired with downstream Seurat and other analytical suites, we turn complex multi-omic matrix sheets into high-fidelity biological landscapes.
Deliverables Include
- FASTQ & Data QC report
- Alignment files (BAM)
- Cell Ranger & Sequencing Saturation
- High-Resolution UMAP / t-SNE Clustering
- Automated Cell-Type & Marker Mapping
- V(D)J Clonal Repertoire & Loupe Files
- Multi-Omic ATAC & GEX Overlays
Scoring Heat Map
Delta Distribution
Clonotype Expansion
Cell Type Annotation
Dimensionality Reduction (UMAP / t-SNE) Cluster Plots
Cell-Type Annotation & Subpopulation Distribution
TCR/BCR Clonal Frequency Repertoire Maps
Cell-to-Cell Interaction
Pathway Analysis
V-J Circos Plot
Applications of 10x Genomics Sequencing
Our high-throughput droplet single-cell workflows empower breakthroughs across diverse fields:
Oncology & TME Profiling
Characterize heterogeneous tumor microenvironments, profile rare immune-infiltrating lineages, and identify low-frequency tumor subclones driving metastatic progression.
Translational Biomarker Screening
Leverage fixed sample multiplexing to track longitudinal drug responses, identify expression shifts in disease cohorts, and map target mechanisms of action.
Neurobiology Diversity
Bypass intact cell limitations by utilizing single-nuclei workflows to map complex glial subdivisions, neuronal subtypes, and spatial tracking profiles within frozen brain tissue.
Resources & Insights
SERVICE OVERVIEW TECH SHEET
10x Genomics Single-Cell Services
Download our service guide to walk through all available 10x Genomics options, sample matrices, and platform configurations.
ANALYSIS OVERVIEW TECH SHEET
10x Genomics Single-Cell Analysis Specifications
Learn more about the primary alignment, custom multi-omic pipelines, and cloud bioinformatics offerings at Signios Bio.
BLOG
Choosing Between 10x Chromium Standard vs. Flex Profiling
A technical guide comparing live-cell inputs against the chemical fixation benefits of the probe-based Chromium Flex assay for fragile and archival FFPE tissues.
Frequently Asked Questions
Everything you need to plan your project
How do I launch a 10x Genomics project?
Simply submit a project request through our web portal. Our scientific applications team will schedule a direct call to map out sample parameters, multiplexing options, and generate your custom estimate.
Which single-cell sequencing technologies do you use?
Signios Bio primarily uses 10x Genomics Chromium platforms for single-cell RNA-seq, immune profiling (TCR/BCR), and ATAC-seq. These platforms support high-throughput, high-resolution data generation.
What starting materials are supported by 10x Genomics services?
We process fresh tissue, dissociated cell suspensions, PBMCs, cryopreserved single cells, flash-frozen tissue pieces, and fixed FFPE blocks/curls.
What are the starting inputs, counts and viability requirements?
- Fresh or FACS-Sorted Cells
- Viability: Greater than 85%
- Cell Count: 500,000–1 million (standard) or more than 1 million (high-throughput)
- Media: 10% FBS or PBS with 1% BSA
- Shipment: Less than 24 hours on ice
- Cryopreserved Cells
- Viability: Greater than 85%
- Cell Count: 1–2 million (standard) or more than 2 million (high-throughput)
- Cryopreservant: CryoStor CS10 or 90% FBS with 10% DMSO
- Shipment: Dry ice
- Fresh Tissue
- Tissue Weight: 50–100 mg
- Storage Media: Miltenyi MACS solution (Cat #130-100-008)
- Automated Dissociation: Available for consistent and reliable preparation
- Shipment: Within 48 hours on ice with cold packs
- Frozen Tissue (Nuclei Only)
- Tissue Weight: 50–100 mg
- Freezing Method: Flash-freeze in isopentane with liquid nitrogen or a precooled cryovial on dry ice
- Shipment: Dry ice
Can I run samples that have been fixed for storage?
Yes. Utilizing the probe-based 10x Genomics Chromium Flex protocol, we seamlessly process formaldehyde-fixed cell suspensions or clinical archival FFPE slide tissues without sacrificing data quality.
Can you profile immune cells at single-cell resolution?
Yes. Signios Bio offers single-cell TCR/BCR sequencing integrated with gene expression profiling—enabling paired chain analysis and immune phenotype mapping of T-cells and B-cells.
How many cells can be profiled per sample?
Typical experiments capture 5,000–10,000 cells per sample, scalable depending on input quality and project design. Subsampling and enrichment are also possible.
Can you help with experimental design and panel selection?
Yes. Signios Bio provides consultative support in sample planning, panel selection (e.g., human vs mouse panels), and protocol optimization to ensure study success.
How long do the single-cell services take?
Projects requiring library prep and sequencing complete in 2–4 weeks depending on sample quality, complexity, and analysis requirements.
What standard bioinformatics data files do you deliver?
We deliver fully demultiplexed, raw FASTQ files, processed Cell Ranger feature-barcode matrices, secondary analysis directories (Loupe Browser files), along with publication-ready UMAP plots and expression data processed through Seurat, R, and other programs.
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Start your single-cell project
Tell us about your cells and research goals. A PhD scientist replies within one business day with the right workflow and a tailored quote.
- US-based, in-house at Foster City, CA
- 10x Genomics Chromium platform
- Single-cell RNA-seq, immune profiling & multiome
Request a quote